Hydrolysis of thioester analogs by rat liver phospholipase A1.
نویسندگان
چکیده
منابع مشابه
Soybean Oil Degumming by Immobilized Phospholipase A1
In the present study, we investigated the ability of an immobilized phospholipase A1 (PLA1) in degumming of soybean oil. The enzyme was immobilized by simple adsorption on bentonite without any further modification. The free and immobilized PLA1 were characterized by Fourier Transform InfraRed (FT-IR) spectroscopy and X-Ray Diffraction (XRD). The immobilizat...
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1. Rat liver cytosol contains a heat-sensitive phospholipase A1 active against phosphatidylethanolamine, 1-acylglycerophosphoethanolamine and, to a very much lesser extent, phosphatidylcholine and phosphatidylinositol. 2. Activity towards a pure phosphatidylethanolamine substrate is invoked by the presence of water-soluble cations that do not precipitate at the pH optimum of the enzyme (9.5). I...
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Retinol acetate hydrolase is localized in rat liver microsomes whereas the retinol palmitate-hydrolyzing activity was found in the nuclear and in the mitochondrial lysosome-rich fractions. Acetone-dried powder from whole rat liver retained all of its retinol palmitate-hydrolyzing activity. Ammonium sulfate fractionation of the acetone-dried powder yielded a partially purified enzyme liberating ...
متن کاملHydrolysis of retinol palmitate by rat liver.
Retinol acetate hydrolase is localized in rat liver microsomes whereas the retinol palmitate-hydrolyzing activity was found in the nuclear and in the mitochondrial lysosome-rich fractions. Acetone-dried powder from whole rat liver retained all of its retinol palmitate-hydrolyzing activity. Ammonium sulfate fractionation of the acetone-dried powder yielded a partially purified enzyme liberating ...
متن کاملPreparation of thioester substrates and development of continuous spectrophotometric assays for phospholipase A1 and monoacylglycerol lipase.
Current assays of phospholipase A1 (EC 3.1.1.32) and monoacylglycerol lipase (EC 3.1.1.23) activities in tissues are discontinuous, laborious, and expensive. Some spectrophotometric substrates were synthesized to alleviate this problem. Thioester analogs of phosphatidylcholine and phosphatidylethanolamine. rac-1,2-S,O-didecanoyl-3-phosphocholine-1-mercapto-2,3-propanediol and rac-1,2-S,O-dideca...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)37657-9